Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay.
نویسندگان
چکیده
A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptococcus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures.
منابع مشابه
مقایسه روش کشت و Multiplex PCR در تشخیص باکتری‌های سخت رشد در بیماران مبتلا به عفونت گوش میانی مراجعه‌کننده به بیمارستان امیر اعلم تهران
Abstract: Aims and scope: Otitis media (OM) is a common disease among children. Various factors, including bacteria and viruses are the cause of OM. The most common bacterial pathogens that can cause OM are Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Streptococcus pyogenes. Recently, Alloiococcus otitidis is known as one of the causes of OM. For some reasons ...
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Background: Streptococcus pneumoniae and Haemophilus influenzae type b (Hib) is a leading cause of bacterial pneumonia, meningitis, and sepsis in children under 5-year of age. This study aims to estimate potentially preventable number of cases and deaths caused by Hib and pneumococcal in Iran.Materials and Methods:We used the burden of diseases model estimates of the World Health Organization (...
متن کاملPolymerase Chain Reaction, Bacteriologic Detection and Antibiogram of Bacteria Isolated from Otitis Media with Effusion in Children, Shiraz, Iran
Background: Otitis media with effusion is one of the leading causes of hearing loss in children. Effective treatment of effusion in the middle ear requires appropriate empirical treatment and characterization of responsible pathogens. Objective of the present study was to detect pathogens in clinical samples from patients with otitis media with effusion in our area and to determine the sensitiv...
متن کاملDETECTION OF HAEMOPHILUS INFLUENZAE TYPE B IN CEREBROSPINAL FLUID OF SUSPECTED CHILDREN WITH MENINGITIS BYPCR
ABSTRACT Background: A polymerase chain reaction (PCR) assay with primers from "hpd" gene was compared with culture for the detection of Haemophilus influenzae type b (Hib) in CSF samples from children suspected of meningitis. Methods: 300 cerebrospinal fluid (CSF) samples from suspected meningitis cases were studied by culture (Leventhal & Chocolate Agar) and PCR. Results: The latter...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 34 8 شماره
صفحات -
تاریخ انتشار 1996